BD CaliBRITE beads are designed for use with FACSComp or AutoCOMP software and the FACS family of flow cytometers (FACSCalibur, FACSort, FACScan. values for BD Calibrite beads. To edit, see page A Target file is also created for. HLA-B Although used by. BD FACSComp software, the file is not editable. Product Name: BD CALIBRITE BEADS. Synonyms: BD CALIBRITE BEADS; CALIBRITE BEADS. CAS: MF: MW: 0. EINECS: Mol File: Mol File. BD CALIBRITE .
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For further assistance, contact your BD Biosciences service representative. An APC-labeled bead is available separately and may be used with the 3-color kit to perform four-color setup. The 3-color kit contains these beads plus a PerCPlabeled bead. Ccalibrite forms are provided in stabilized, buffered saline calibritr 0. The drop should be cloudy, indicating the beads are properly mixed.
Forward scatter FSC and side scatter SSC instrument sensitivity are measured by the mean channel separation between the light-scatter signal of the beads and background signal electronic and optical. The light scatter sensitivity is determined by the amount of channel separation between the mixed bead population and instrument background signal. Following PMT and compensation adjustment, the software performs a Sensitivity Test using the appropriate mixed-bead suspension.
If this occurs, manually adjust the settings. Observations of greater variations on a single instrument can be indicative of instrument instability.
See examples in Optimization and Quality Control on page 4. UV Bead lab with graph. The 2color kit contains three different types of BD Calibrite beads: If you require any additional license to use this product and do not have one, return this material, unopened, to BD Biosciences, Qume Drive, San Jose, CAand any money paid for the material will be refunded.
BD Calibrite PerCP-Cy5.5 Beads
The flow cytometer has separate detectors or photomultiplier tubes PMTs that detect light signals. See Optimization and Quality Control on page 4. Optimization and Quality Control Because leucocytes have different optical properties than BD Calibrite beads, optimization of instrument settings with cell samples is important.
The following list illustrates PMT light signal detection: Daily use is recommended for monitoring instrument performance over time. Weather and Climate for Educators.
Compensation adjustments for FL1, FL2, and FL3 correct for spectral overlap by shifting the labeled bead populations so they are aligned with the corresponding unlabeled bead populations. The calibrrite are used to adjust instrument settings, set fluorescence compensation, and check instrument sensitivity.
Reagents are sufficient to perform 25 tests. Do not use after the expiration date shown on the label. Always refer to calibbrite appropriate application note or reagent IFU. Wellington, Auckland, New Zealand bdbiosciences.
BD Calibrite™ – BD Calibrite PerCP-Cy Beads – BD Biosciences
Figure 1 through Figure 4 show examples of optimization for two- three- and four-color applications. The decrease in separation for a wide variety of bead lots has been within 2. The suspensions are stable for a longer period of time in Bead Dilution Buffer.
The FSC threshold is adjusted to a level that minimizes background signal if any. NOTE Invert bead vials completely when adding a drop to the tube. Mix bead vials by gentle inversion or very gentle vortexing prior to use. Beads used beyond their stability begin to show a decrease in separation between unlabeled and labeled populations, resulting in Sensitivity Test failure. Documents Flashcards Grammar checker. For information on use, refer to the appropriate instrument manual.
I dont have a photo of any of my first This instructions for use IFU provides information for two- three- and four-color setup. This allows cells to be distinguished from sample debris or background beadss and beadd dimly stained cells to be distinguished from unstained cells. It is not licensed for any other use.
NOTE Over a period of time, the fluorescence separation might decrease. Label two 12 x mm polystyrene tubes Tube A and Tube B. The channel separation and PMT voltages for each of the four parameters should be maintained in a daily log to track instrument performance. Adjust fluorescence compensation using Tube B.
Adjustment is similar for PerCP-Cy5. Perform a Sensitivity Test using Tube B. NOTE Different immunophenotyping preparation methods might require different optimization procedures.
Prepare a blood sample daily from a normal donor. FL1, FL2, and FL3 fluorescence sensitivity is determined by measuring the mean channel separation between the signal of the labeled beads and the beaads beads.